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polyclonal rabbit anti-human β 2 m (batch 5511)  (Absolute Biotech Inc)

 
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    Absolute Biotech Inc polyclonal rabbit anti-human β 2 m (batch 5511)
    Comparison of 6 μm microcapsules and 2.35 μm PS beads for binding of antibody and for the detection of a protein analyte: (A+B) Detection of the capture antibody, BBM.1, on microcapsules and beads with fluorescently labeled goat anti-mouse antibody (GαM-AF488) by flow cytometry. (A) shows a representative experiment, (B) the average of two experiments with standard deviations (SD). (C) Schematics for the detection of human beta-2 microglobulin (hβ 2 m). The capture antibody, BBM.1, is immobilized on the protein A-coated microcapsules/PS beads. BBM.1 antibody binds specifically to hβ 2 m, which is sandwiched by the <t>polyclonal</t> rabbit anti-hβ 2 M (Rαhβ 2 M) antibody. The sandwich is then detected by adding AF488 labeled goat anti-rabbit (GαR-AF488) antibody. (D) Detection of hβ 2 m in PBS. Dose-response curves for the assay performed as in (C) with microcapsules or PS beads. MFI values are normalized to the maximum values. Error bars are SD (n = 3). Invisible error bars are smaller than the size of the marker. (E) Control samples of hβ 2 m plotted as histograms. Experiments were performed as in (C). Samples with analyte (10 5 pg mL -1 for microcapsules and 10 6 pg mL -1 for PS beads) were used as positive control and for normalization, which was done individually for microcapsules and PS beads. Error bars are SD (n = 3).
    Polyclonal Rabbit Anti Human β 2 M (Batch 5511), supplied by Absolute Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+rabbit+anti-human+%CE%B2+2+m+(batch+5511)/polyclonal+rabbit+anti+human+%CE%B2+2+m++batch+5511+/pmc06054379-48-24-35
    Average 90 stars, based on 1 article reviews
    polyclonal rabbit anti-human β 2 m (batch 5511) - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids"

    Article Title: Comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0201009

    Comparison of 6 μm microcapsules and 2.35 μm PS beads for binding of antibody and for the detection of a protein analyte: (A+B) Detection of the capture antibody, BBM.1, on microcapsules and beads with fluorescently labeled goat anti-mouse antibody (GαM-AF488) by flow cytometry. (A) shows a representative experiment, (B) the average of two experiments with standard deviations (SD). (C) Schematics for the detection of human beta-2 microglobulin (hβ 2 m). The capture antibody, BBM.1, is immobilized on the protein A-coated microcapsules/PS beads. BBM.1 antibody binds specifically to hβ 2 m, which is sandwiched by the polyclonal rabbit anti-hβ 2 M (Rαhβ 2 M) antibody. The sandwich is then detected by adding AF488 labeled goat anti-rabbit (GαR-AF488) antibody. (D) Detection of hβ 2 m in PBS. Dose-response curves for the assay performed as in (C) with microcapsules or PS beads. MFI values are normalized to the maximum values. Error bars are SD (n = 3). Invisible error bars are smaller than the size of the marker. (E) Control samples of hβ 2 m plotted as histograms. Experiments were performed as in (C). Samples with analyte (10 5 pg mL -1 for microcapsules and 10 6 pg mL -1 for PS beads) were used as positive control and for normalization, which was done individually for microcapsules and PS beads. Error bars are SD (n = 3).
    Figure Legend Snippet: Comparison of 6 μm microcapsules and 2.35 μm PS beads for binding of antibody and for the detection of a protein analyte: (A+B) Detection of the capture antibody, BBM.1, on microcapsules and beads with fluorescently labeled goat anti-mouse antibody (GαM-AF488) by flow cytometry. (A) shows a representative experiment, (B) the average of two experiments with standard deviations (SD). (C) Schematics for the detection of human beta-2 microglobulin (hβ 2 m). The capture antibody, BBM.1, is immobilized on the protein A-coated microcapsules/PS beads. BBM.1 antibody binds specifically to hβ 2 m, which is sandwiched by the polyclonal rabbit anti-hβ 2 M (Rαhβ 2 M) antibody. The sandwich is then detected by adding AF488 labeled goat anti-rabbit (GαR-AF488) antibody. (D) Detection of hβ 2 m in PBS. Dose-response curves for the assay performed as in (C) with microcapsules or PS beads. MFI values are normalized to the maximum values. Error bars are SD (n = 3). Invisible error bars are smaller than the size of the marker. (E) Control samples of hβ 2 m plotted as histograms. Experiments were performed as in (C). Samples with analyte (10 5 pg mL -1 for microcapsules and 10 6 pg mL -1 for PS beads) were used as positive control and for normalization, which was done individually for microcapsules and PS beads. Error bars are SD (n = 3).

    Techniques Used: Binding Assay, Labeling, Flow Cytometry, Marker, Positive Control



    Similar Products

    polyclonal rabbit anti-human β 2 m (batch 5511)  (Absolute Biotech Inc)
    90
    Absolute Biotech Inc polyclonal rabbit anti-human β 2 m (batch 5511)
    Comparison of 6 μm microcapsules and 2.35 μm PS beads for binding of antibody and for the detection of a protein analyte: (A+B) Detection of the capture antibody, BBM.1, on microcapsules and beads with fluorescently labeled goat anti-mouse antibody (GαM-AF488) by flow cytometry. (A) shows a representative experiment, (B) the average of two experiments with standard deviations (SD). (C) Schematics for the detection of human beta-2 microglobulin (hβ 2 m). The capture antibody, BBM.1, is immobilized on the protein A-coated microcapsules/PS beads. BBM.1 antibody binds specifically to hβ 2 m, which is sandwiched by the <t>polyclonal</t> rabbit anti-hβ 2 M (Rαhβ 2 M) antibody. The sandwich is then detected by adding AF488 labeled goat anti-rabbit (GαR-AF488) antibody. (D) Detection of hβ 2 m in PBS. Dose-response curves for the assay performed as in (C) with microcapsules or PS beads. MFI values are normalized to the maximum values. Error bars are SD (n = 3). Invisible error bars are smaller than the size of the marker. (E) Control samples of hβ 2 m plotted as histograms. Experiments were performed as in (C). Samples with analyte (10 5 pg mL -1 for microcapsules and 10 6 pg mL -1 for PS beads) were used as positive control and for normalization, which was done individually for microcapsules and PS beads. Error bars are SD (n = 3).
    Polyclonal Rabbit Anti Human β 2 M (Batch 5511), supplied by Absolute Biotech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+rabbit+anti-human+%CE%B2+2+m+(batch+5511)/polyclonal+rabbit+anti+human+%CE%B2+2+m++batch+5511+/pmc06054379-48-24-35
    Average 90 stars, based on 1 article reviews
    polyclonal rabbit anti-human β 2 m (batch 5511) - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Comparison of 6 μm microcapsules and 2.35 μm PS beads for binding of antibody and for the detection of a protein analyte: (A+B) Detection of the capture antibody, BBM.1, on microcapsules and beads with fluorescently labeled goat anti-mouse antibody (GαM-AF488) by flow cytometry. (A) shows a representative experiment, (B) the average of two experiments with standard deviations (SD). (C) Schematics for the detection of human beta-2 microglobulin (hβ 2 m). The capture antibody, BBM.1, is immobilized on the protein A-coated microcapsules/PS beads. BBM.1 antibody binds specifically to hβ 2 m, which is sandwiched by the polyclonal rabbit anti-hβ 2 M (Rαhβ 2 M) antibody. The sandwich is then detected by adding AF488 labeled goat anti-rabbit (GαR-AF488) antibody. (D) Detection of hβ 2 m in PBS. Dose-response curves for the assay performed as in (C) with microcapsules or PS beads. MFI values are normalized to the maximum values. Error bars are SD (n = 3). Invisible error bars are smaller than the size of the marker. (E) Control samples of hβ 2 m plotted as histograms. Experiments were performed as in (C). Samples with analyte (10 5 pg mL -1 for microcapsules and 10 6 pg mL -1 for PS beads) were used as positive control and for normalization, which was done individually for microcapsules and PS beads. Error bars are SD (n = 3).

    Journal: PLoS ONE

    Article Title: Comparative validation of a microcapsule-based immunoassay for the detection of proteins and nucleic acids

    doi: 10.1371/journal.pone.0201009

    Figure Lengend Snippet: Comparison of 6 μm microcapsules and 2.35 μm PS beads for binding of antibody and for the detection of a protein analyte: (A+B) Detection of the capture antibody, BBM.1, on microcapsules and beads with fluorescently labeled goat anti-mouse antibody (GαM-AF488) by flow cytometry. (A) shows a representative experiment, (B) the average of two experiments with standard deviations (SD). (C) Schematics for the detection of human beta-2 microglobulin (hβ 2 m). The capture antibody, BBM.1, is immobilized on the protein A-coated microcapsules/PS beads. BBM.1 antibody binds specifically to hβ 2 m, which is sandwiched by the polyclonal rabbit anti-hβ 2 M (Rαhβ 2 M) antibody. The sandwich is then detected by adding AF488 labeled goat anti-rabbit (GαR-AF488) antibody. (D) Detection of hβ 2 m in PBS. Dose-response curves for the assay performed as in (C) with microcapsules or PS beads. MFI values are normalized to the maximum values. Error bars are SD (n = 3). Invisible error bars are smaller than the size of the marker. (E) Control samples of hβ 2 m plotted as histograms. Experiments were performed as in (C). Samples with analyte (10 5 pg mL -1 for microcapsules and 10 6 pg mL -1 for PS beads) were used as positive control and for normalization, which was done individually for microcapsules and PS beads. Error bars are SD (n = 3).

    Article Snippet: Polyclonal goat anti-mouse antibody (Cat. No. A11001) and goat anti-rabbit antibody (Cat. No. A11008) labeled with Alexa Fluor 488 were purchased from Invitrogen, and polyclonal rabbit anti-human β 2 m (Batch 5511) was purchased from Nordic Immunology.

    Techniques: Binding Assay, Labeling, Flow Cytometry, Marker, Positive Control